Method for microRNA isolation from clinical serum samples
نویسندگان
چکیده
منابع مشابه
A microRNA isolation method from clinical samples
INTRODUCTION microRNAs (miRNAs) are considered to be novel molecular biomakers that could be exploited in the diagnosis and treatment of different diseases. The present study aimed to develop an efficient miRNA isolation method from different clinical specimens. METHODS Total RNAs were isolated by Trizol reagent followed by precipitation of the large RNAs with potassium acetate (KCH3COOH), po...
متن کاملImproved microRNA quantification in total RNA from clinical samples.
microRNAs are small regulatory RNAs that are currently emerging as new biomarkers for cancer and other diseases. In order for biomarkers to be useful in clinical settings, they should be accurately and reliably detected in clinical samples such as formalin fixed paraffin embedded (FFPE) sections and blood serum or plasma. These types of samples represent a challenge in terms of microRNA quantif...
متن کاملoptimizing microrna quantification in serum samples
micrornas constitute a group of small non-coding rnas that negatively regulate gene expression. aside from their contribution to biological and pathological pathways, altered expression of micrornas is reported in bio-fluid samples, such as serum. to employ serum's micrornas as potential biomarkers, it is crucial to develop an efficient method for microrna quantification, avoiding pre-analytica...
متن کاملIsolation and Characterization of Novel Human Parechovirus from Clinical Samples
Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. Neutralization test showed that the NII561-2000 virus had distinct antigenicity to HPeV-1, HPeV-2, and HPeV-3, and that the sequence...
متن کاملIsolation of Legionella pneumophila from Tehran Hospital Water Samples
Over a Period of three months (summer), 108 samples of water collected from four hospitals in Te-hran. They were taken from numerous potable and non-potable water, including main supply cis-terns, calorifiers, hot and cold tap water, shower, cooling tower and drain of air condition system. Culturing of concentrated and non-concentrated water samples on Buffered Charcoal Yeast Extract (BCYE) Aga...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2012
ISSN: 0003-2697
DOI: 10.1016/j.ab.2012.09.007